Allele-Specific Conditional Destabilization of Glutamine Repeat mRNAs

Heat shock causes destabilization of specific mRNAs and destruction of endoplasmic reticulum in barley aleurone cells.

In response to a phytohormone, gibberellic acid, the aleurone layers of barley seeds synthesize and secrete alpha-amylases, which are coded by a set of stable mRNAs. When aleurone layers are subjected to heat shock treatment, the synthesis of alpha-amylase is suppressed while heat shock proteins are induced. The suppression of alpha-amylase synthesis is not the result of translational control a...

Application of Allele Specific Markers in Determining Alleles at VRN-1 Loci in Bread Wheat Genotypes

    Wheat is the most important crop and is cultivated all over the world. Wheat has a wide range of adaptability to different climates. Vernalization genes are one of the important factors determining wheat adaptability. in wheat breeding program understanding the alleles at vernalization requirement gens are useful to introduce new cultivar for different climates. At the molecular level, the ...

Allele-Specific Gene Silencing

Cardiac arrhythmias are a major healthcare problem in the developed world. The American Heart Association 2017 statistical report indicates that atrial fibrillation, the most common cardiac rhythm disorder, has an ≈25% lifetime incidence and annual costs of ≈26 billion dollars, whereas sudden cardiac death (usually caused by malignant arrhythmias) affects fewer individuals (just under 200 000 A...

Allele-Specific Regulation of TCR

When more is less: Pathogenesis of glutamine repeat neurodegenerative diseases

Five neurodegenerative diseases are caused by expanding CAG triplet repeats coding for polyglutamine. They all involve loss of selected populations of neurons, usually in adulthood. How the genetic lesions cause progressive neuronal degeneration is unknown. Unraveling the pathogenesis of these disorders will help elucidate pathways of neuronal cell death. Triplet repeat disorders involve the re...